42364
Sigma
1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate
BioChemika, for fluorescence, ≥98.0% (TLC)
| Synonym: | DiI |
| CAS Number: | 41085-99-8 |
| Linear Formula: | C59H97ClN2O4 |
| Molecular Weight: | 933.87 |
| Beilstein Registry Number: | 8251870 |
| MDL number: | MFCD00142354 |
| PubChem Substance ID: | 24866515 |
Description
| Analysis Note | In addition to the emission maximum at 565 nm, there is lower maximum at ~605 nm. |
| Biochem/physiol Actions | Lipophilic carbocyanine dye used primarily for optical recordings of membrane voltage and for studies of membrane fluidity. Retrograde stain for neurons; provides intense, long-lasting staining of live neurons in vivo and in vitro. |
| Other Notes | Lipophilic fluorescent probe for studying membranes1,2 |
Properties
| grade | for fluorescence |
| product line | BioChemika |
| assay | ≥98.0% (TLC) |
| fluorescence | λex 550 nm; λem 565 nm in phosphate buffer/SDS pH 7.0 |
| mp | 68 °C (dec.)(lit.) |
| solubility | DMF: soluble |
| DMSO: soluble | |
| methanol: soluble |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| Cited Reference | 1. R.D. Klausner, D.E. Wolf Biochemistry 19, 6199, (1980) Abstract |
| 2. M.F. Ethier et al. Biochemistry 22, 1178, (1983) Abstract | |
| reference | Godement, P., et al., A study in developing visual systems with a new method of staining neurons and their processes in fixed tissue. Development 101, 697, (1987) |
| Heimer, L., ed., Zaborszky, L., ed. Neuroanatomical Tract-tracing Methods 2nd ed., New York , (1989) |
